ISO 20776-1 PDF

ISO. First edition. Clinical laboratory testing and in vitro diagnostic test systems — Susceptibility testing of infectious agents and evaluation. Buy ISO , Susceptibility testing of infectious agents and evaluation of performance of antimicrobial susceptibility test devices, Part 1, at CLSI. ISO () Clinical laboratory testing and in vitro diagnostic test systems – Susceptibility testing of infectious agents and evaluation of performance of.

Author: Zoloran Garamar
Country: Benin
Language: English (Spanish)
Genre: Politics
Published (Last): 16 December 2011
Pages: 293
PDF File Size: 7.6 Mb
ePub File Size: 2.80 Mb
ISBN: 725-6-50650-485-8
Downloads: 22446
Price: Free* [*Free Regsitration Required]
Uploader: Dailabar

Rationale documents for antifungals Evaluation ieo performance of antimicrobial susceptibility test devices. Breakpoints for short incubation Expert rules and intrinsic resistance Resistance mechanisms Guidance documents Consultations – New!

BS EN ISO 20776-1:2006

A multicenter study was conducted to assess the accuracy of the ISO standard and the serial 2-fold dilution procedures for antifungal susceptibility testing.

Journal List J Clin Microbiol v.

Contact Sitemap Privacy Statement Disclaimer. All statistical analyses were done with the statistical package PAWS statistics Clinical laboratory 20776-1 and in vitro diagnostic test systems. In summary, our data suggest that microdilution trays for hydrophilic drugs such as FLC can be accurately prepared following the ISO recommendations or the serial 2-fold dilution schemes.

Precision of fluconazole dilution series by dilution procedure and laboratory a. Fifty microliters of the resultant mix was injected directly into the HPLC system. Clinical and Laboratory Standards Institute.


EUCAST: External documents

However, itraconazole trays showed a significant lack of reproducibility that was independent of which method was followed. Development and validation of a high-performance liquid chromatographic assay for the determination of fluconazole in human whole blood using solid phase extraction. Comparisons between results obtained by the tray preparation method were done by using analysis of variance ANOVA. HPLC is a sensitive and reproducible method for determining levels of azole components in a great variety of matrices, as has been confirmed in previous reports 12510 – 1215lso – 21 The broth microdilution method described in this part of ISO is essentially the same as those used in many countries, including France, Germany, Sweden, the United Kingdom, and the United States.

I agree More information. Support Center Support Center. National Center for Biotechnology InformationU. Single-step extraction of fluconazole from plasma by ultra-filtration for the measurement of its free concentration by high performance liquid chromatography. First indications of improved capability and capacity of public health microbiology laboratories in the European Union http: You are advised 207761 check costs as they may differ substantially between suppliers.

Relevant external documents

Empfindlichkeitspruefung von Infektionserregern und Evaluation von Geraeten zur antimikrobiellen Empfindlichkeitspruefung. The method was validated by performing three calibration curves on consecutive days.

Susceptibility testing of infectious agents and evaluation of performance of antimicrobial susceptibility testing devices. Selective high-performance liquid chromatographic assay for itraconazole and hydroxyitraconazole in plasma from human immunodeficiency virus-infected patients.


ECDC roadmap for integration of ios typing and genomic typing into European-level surveillance and epidemic preparedness — Version 2. In dilution tests, microorganisms are tested for their ability to produce visible growth on a series of agar plates agar dilution or in broth broth dilution containing serial dilutions of the antimicrobial agent.

Reference method for testing the in vitro activity of antimicrobial agents against rapidly growing aerobic bacteria involved in infectious diseases Status Current, Work in hand Publication Date 29 December Cross References DIN Published online Mar Meetings, Minutes and Reports All these methods are based on those described by Ericsson and Sherris.

Susceptibility testing of pathogenic fungi with itraconazole: Regarding serial 2-fold dilution procedures, better results were detected uso FLC plates were prepared according to the hydrophilic rather than the hydrophobic way. The tests are also important in resistance surveillance, epidemiological studies of susceptibility and in comparisons of new and existing agents.

Development and validation of an efficient HPLC method for quantification of voriconazole in plasma and microdialysate reflecting an important target site.